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Image Search Results
Journal: bioRxiv
Article Title: A symbiotic MLO gene regulates root development via RALF34-triggered Ca 2+ signalling in Lotus japonicus
doi: 10.1101/2025.09.18.676995
Figure Lengend Snippet: E. coli cells were co-transformed with pET-AEQ for expression of aequorin and pRham. Three different versions of pRham were used: the empty vector as control (empty), the vector containing LjMLO4 CDS optimized for expression in E. coli (MLO4, 65 kDa) and a truncated version of the gene (AA 1-265, MLO4Δ, 31 kDa) mimicking the putative truncated protein in mlo4-3 L. japonicus mutant line. A) Schematic representation of the two versions of LjMLO4 expressed in E. coli. The representative image of MLO topology (modified from Kusch et al . 2016 ) highlights the missing domains in LjMLO4Δ. B) Western blot showing the expression of LjMLO4-HIS and LjMLO4Δ-HIS in E. coli cells using anti-HIS antibody. D-G) Ca 2+ influx after injection of 1 mM CaCl 2 represented as Ca 2+ traces along time (D), level of Ca 2+ after 2 minutes (E) and after 3 minutes (F), total Ca 2+ mobilized over time (G). Statistical analysis was conducted via Kruskal-Wallis test followed by Dunn’s post-hoc correction (only p-values < 0.1 are reported).
Article Snippet: 6xHis-tagged proteins were detected by overnight incubation at 4 °C with
Techniques: Transformation Assay, Expressing, Plasmid Preparation, Control, Mutagenesis, Modification, Western Blot, Injection